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Protocols

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Assay pipeline

  • Protocols:RNA_extraction
  • Protocols:HeliScopeCAGE
  • Protocols:Low_quantity_HeliScopeCAGE
  • Protocols:SmallRNAseq
  • Protocols:RNAseq

Computational Pipeline

  • Protocols:rRNAdust
  • Protocols:HeliScopeCAGE_read_alignment - delve, filtering (Q20 + percent identity), CTSS
  • Protocols:RNAseq_read_alignment - TopHat, Cufflinks, (Trinity?), Cuffmerge
  • Protocols:SmallRNAseq_read_alignment - BWA

Computational analysis

  • Protocols:CAGE_peak_calling
  • Protocols:GOstat_on_coexpression_cluster
  • Protocols:GOstat_on_MARA_motif_target
  • Protocols:Sample_enrichment_analysis_on_CAGE_peak_expression
  • Protocols:Sample_enrichment_analysis_on_coexpression_cluster
  • Protocols:Motif_Activity_Response_Analysis(MARA)