Rinderpest infection series

Series:	IN_VITRO DIFFERENTIATION SERIES
Species:	Human (Homo sapiens)
Genomic View:	Zenbu
Expression table:	FILE
Link to TET:	TET
Sample providers :	Michiko Kai
Germ layer:	mesoderm
Primary cells or cell line:	primary cells
Time span:	48 hours
Number of time points:	5

Overview

Morbilliviruses including measles virus and rinderpest virus (RPV) are one of the most important pathogens in their respective hosts. In particular, transient strong immunosuppression is the most characteristic feature. Morbillivirus infection induces cell-type-dependent immune responses. However, the molecular mechanisms have not been elucidated. Morbillivirus possesses two nonstructural accessory proteins, V and C. These proteins have been shown to inhibit interferon induction and signaling, although their full functions are unclear. To resolve the complexity and multidimensionality of virus–host interactions, we established recombinant RPV that lacked V and C proteins, and searched for the cell-type-specific transcriptional regulatory network after infection with the FANTOM5 time course analysis. Our study provides a new insight that an accessory protein is one of the virulence factors that define a cell-type-specific response of morbillivirus. This high-throughput experiment uncovers a novel host response against virus infection, and will be useful for understanding the whole picture of virus pathogenesis.

Sample description

We used two major target cell type for RPV infection, a lymphoid cell line (COBL-a cells) and an epithelial cell line (HEK293 cells stably expressing receptor SLAM; 293SLAM cells) [1]. In addition, we established a reverse genetics for RPV-L strain, which is highly virulent against rabbit [2,3], and succeeded in generating recombinant RPV lacking C protein. Cells were infected with each RPV at a multiplicity of infection of 2, and were harvested at 6, 12, 24 and 48 h post infection. Three biological triplicates were performed. References: [1] Measles virus induces cell-type specific changes in gene expression. Sato H1, Honma R, Yoneda M, Miura R, Tsukiyama-Kohara K, Ikeda F, Seki T, Watanabe S, Kai C. Virology. 2008 Jun 5;375(2):321-30. PMID:18374960 [2] Rinderpest virus H protein: role in determining host range in rabbits. Yoneda M, Bandyopadhyay SK, Shiotani M, Fujita K, Nuntaprasert A, Miura R, Baron MD, Barrett T, Kai C. J Gen Virol. 2002 Jun;83(Pt 6):1457-63. PMID:12029161 [3] Rinderpest virus phosphoprotein gene is a major determinant of species-specific pathogenicity. Yoneda M, Miura R, Barrett T, Tsukiyama-Kohara K, Kai C. J Virol. 2004 Jun;78(12):6676-81. PMID:15163758

Quality control
No marker genes are established yet. Infection, replication and growth of virus were verified by cytopathic effect of cells (formation of multinuclear giant cells).

Profiled time course samples

Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples

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13541-145H4	293SLAM rinderpest infection	00hr	biol_rep1
13542-145H5	293SLAM rinderpest infection	00hr	biol_rep2
13543-145H6	293SLAM rinderpest infection	00hr	biol_rep3
13544-145H7	293SLAM rinderpest infection	06hr	biol_rep1
13545-145H8	293SLAM rinderpest infection	06hr	biol_rep2
13546-145H9	293SLAM rinderpest infection	06hr	biol_rep3
13547-145I1	293SLAM rinderpest infection	12hr	biol_rep1
13548-145I2	293SLAM rinderpest infection	12hr	biol_rep2
13549-145I3	293SLAM rinderpest infection	12hr	biol_rep3
13550-145I4	293SLAM rinderpest infection	24hr	biol_rep1
13551-145I5	293SLAM rinderpest infection	24hr	biol_rep2
13552-145I6	293SLAM rinderpest infection	24hr	biol_rep3
13553-145I7	COBL-a rinderpest infection	00hr	biol_rep1
13554-145I8	COBL-a rinderpest infection	00hr	biol_rep2
13555-145I9	COBL-a rinderpest infection	00hr	biol_rep3
13556-146A1	COBL-a rinderpest infection	06hr	biol_rep1
13557-146A2	COBL-a rinderpest infection	06hr	biol_rep2
13558-146A3	COBL-a rinderpest infection	06hr	biol_rep3
13559-146A4	COBL-a rinderpest infection	12hr	biol_rep1
13560-146A5	COBL-a rinderpest infection	12hr	biol_rep2
13561-146A6	COBL-a rinderpest infection	12hr	biol_rep3
13562-146A7	COBL-a rinderpest infection	24hr	biol_rep1
13563-146A8	COBL-a rinderpest infection	24hr	biol_rep2
13564-146A9	COBL-a rinderpest infection	24hr	biol_rep3
13565-146B1	COBL-a rinderpest infection	48hr	biol_rep1
13566-146B2	COBL-a rinderpest infection	48hr	biol_rep2
13567-146B3	COBL-a rinderpest infection	48hr	biol_rep3
13568-146B4	COBL-a rinderpest(-C) infection	06hr	biol_rep1
13569-146B5	COBL-a rinderpest(-C) infection	06hr	biol_rep2
13570-146B6	COBL-a rinderpest(-C) infection	06hr	biol_rep3
13571-146B7	COBL-a rinderpest(-C) infection	12hr	biol_rep1
13572-146B8	COBL-a rinderpest(-C) infection	12hr	biol_rep2
13573-146B9	COBL-a rinderpest(-C) infection	12hr	biol_rep3
13574-146C1	COBL-a rinderpest(-C) infection	24hr	biol_rep1
13575-146C2	COBL-a rinderpest(-C) infection	24hr	biol_rep2
13576-146C3	COBL-a rinderpest(-C) infection	24hr	biol_rep3
13577-146C4	COBL-a rinderpest(-C) infection	48hr	biol_rep1
13578-146C5	COBL-a rinderpest(-C) infection	48hr	biol_rep2
13579-146C6	COBL-a rinderpest(-C) infection	48hr	biol_rep3