MCF7 response to HRG: Difference between revisions
From FANTOM5_SSTAR
No edit summary |
No edit summary |
||
| Line 14: | Line 14: | ||
|series=IN_VITRO DIFFERENTIATION SERIES | |series=IN_VITRO DIFFERENTIATION SERIES | ||
|species=Human (Homo sapiens) | |species=Human (Homo sapiens) | ||
|tet_config= | |tet_config=https://fantom.gsc.riken.jp/5/suppl/tet/MCF7_breast_cancer_cell_line_HRG.tsv.gz | ||
|tet_file= | |tet_file=https://fantom.gsc.riken.jp/5/tet#!/search/?filename=hg19.cage_peak_phase1and2combined_tpm_ann_decoded.osc.txt.gz&file=1&c=1&c=682&c=683&c=684&c=729&c=730&c=731&c=732&c=733&c=734&c=735&c=736&c=737&c=738&c=739&c=740&c=741&c=742&c=743&c=744&c=745&c=746&c=747&c=748&c=749&c=750&c=751&c=752&c=753&c=754&c=755&c=756&c=757&c=758&c=759&c=760&c=761&c=762&c=763&c=764&c=765&c=766&c=767&c=768&c=769&c=770&c=771&c=772&c=773 | ||
|time_points=0hr | |time_points=0hr | ||
|time_span=8 hours | |time_span=8 hours | ||
|timepoint_design=Early focus | |timepoint_design=Early focus | ||
|tissue_cell_type=Breast epithelia cells | |tissue_cell_type=Breast epithelia cells | ||
|zenbu_config= | |zenbu_config=https://fantom.gsc.riken.jp/zenbu/gLyphs/#config=9bzxFdkiAVkjy3T1p86nGD | ||
}} | }} | ||
Latest revision as of 17:18, 14 March 2022
| Series: | IN_VITRO DIFFERENTIATION SERIES |
|---|---|
| Species: | Human (Homo sapiens) |
| Genomic View: | Zenbu |
| Expression table: | FILE |
| Link to TET: | TET |
| Sample providers : | Mariko Okada |
| Germ layer: | ectoderm |
| Primary cells or cell line: | cell line |
| Time span: | 8 hours |
| Number of time points: | 16 |
| CollapseOverview |
|---|
|
ErbB receptor family plays a central role in cellular development, proliferation, differentiation and cell death. There are four members, ErbB1/EGFR, ErbB2, ErbB3 and ErbB4 receptors, belong to this family. Dysregulation of the receptors is highly associated with incidence of variety of cancers [1]. Once activated by growth factor binding, the receptor forms homo- and heterodimer and are trans-activated by tyrosine phosphorylation, transmits the phosphorylation signal to downstream kinase such as ERK and Akt, then those kinases activate/phosphorylate transcription factors such as ELK1, CREB, or SRF in nucleus to initiate gene expression.In human breast cancer MCF-7 cells, the heregulin (HRG; a ErbB3/4 ligand) induces cell differentiation (accumulation of lipid droplets), while epidermal growth factor (EGF; an ErbB1/EGFR ligand) elicits cell proliferation after 5-14 days of the ligand stimulation.These ligand-stimulated cells show similar time-course profiles in terms of signaling activity and immediate early gene (IEG) mRNA expression (up to 1.5 hours)[2,3]. However, analysis of delayed transcription (up to 72 hours) using qRT-PCR showed the significant expression of transcription factors (c-FOS, FRA-1 and FHL2) specific to HRG but not by EGF [4], suggesting the existence of unknown transcription machinery for cell differentiation during this time-course. |
Figure 1: Approximate expression timing of early, mid, delayed response genes.| ExpandSample description |
|---|
| ExpandQuality control |
|---|
The mRNA expression of c-FOS, FOSL1 and FHL2 was significantly induced for HRG than EGF shown in the past study (The figures from Saeki et al BMC Genomics 2009[4]).
Time-course patterns the CAGE peaks of c-FOS, FOSL1, FHL2 obtained from current analysis was consistent with the mRNA expression data shown in above.Profiled time course samples
Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples
