Vector
phage vector plasmid vector
(before excision (local))		 original vector
λ-modified Bluescript1 modified pBluescript SK(+)/modified Bluescript1 pBluescript SK(+)
λ-FLCI pFLCI pBluescript KS(+)
λ-FLCII pFLCII pBluescriptII SK(+)
λ-FLCIII(λ-FLCIII-L) pFLCIII pBluescript KS(+)

More info...STRATAGENE --pBluescriptII (PDF file)

Notes: Both "λ-FLCIII" and "λ-FLCIII-L" change in "pFLCIII".

After Excision
vector CODE
of rearray		 1st-primer for
cDNA construction		 2nd-primer for
cDNA construction		 g-tail poly-A
modified Bluescript1 ZA 1st-NX 2nd-AT + + rough sketch detail
modified Bluescript1 ZX 1st-BS 2nd-NX/X + + rough sketch detail
pFLCI PX
PY(strain="NOD")
PZ(host="DH5a")		 1st-BS 2nd-NX/X + + rough sketch detail
pFLCI PK
PT(1st-primer="1st-BG tag", strain="Transgenic hTNF")
PI(1st-primer="1st-BG tag", strain="CD-1")
PL(1st-primer="1st-BG tag")		 1st-BS 2nd-linker - + rough sketch detail
pFLCI PH(1st-primer="1st-BG tag") 1st-BS 2nd-hairpin-linker - + rough sketch detail
pFLCI PR
PN(strain="NOD")		 1st-BG 2nd-linker - + rough sketch detail
pFLCI PS
PM(strain="NOD") 		1st-BG(short Poly-A) 2nd-linker - short rough sketch detail
pFLCII FX 1st-BS 2nd-NX/X + + rough sketch detail
pDEST FX (libraryid=E8,E9) 1st-BS 2nd-NX/X + + rough sketch -
pFLCII FK 1st-BS 2nd-linker - + rough sketch detail
pDEST FK (libraryid=E8,E9) 1st-BS 2nd-linker - + rough sketch -
pFLCII FL 1st-BG(short Poly-A) 2nd-linker - short rough sketch detail
pFLCIII FU
FV(1st-primer="1st-BG tag")		 1st-BS 2nd-NX/X + + - detail
pFLCIII FS(1st-primer="1st-BG tag" 1st-BS 2nd-linker - + - detail
pFLCIII FT(1st-primer="1st-BG tag") 1st-BS 2nd-hairpin-linker - + - detail
pFLCIII-L(pBAC) FW(1st-primer="1st-BG tag")
FY(1st-primer="1st-BG tag", host="JW366")		 1st BG tag 2nd linker - - rough sketch -

Notes: 1st-BG tagの場合、配列は1st-"BS"のものを参照してください。(SstI部分の配列が異なります。)
(Notes: "1st-BS" and "1st-BG tag" are almost the same. However, the part of "SstI" site is different. )

(1st-BG tag (for mixed library))
name site sequence length
1st-BG tag BamHI,
(SstI(NNNNNN=GAGCTC))		 5'-GAGAGAGAGAAGGATCCAA(NNNNNN)TTTTTTTTTTTTTTTTVN-3' 43mer
Notes : V=A,G,C N=A,G,C,T
1st BS = 1st tag A01 (GAGCTC)(BamHI,SstI)